| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cai, Cynthia D (12)
|
MRRA |
33.27 |
32.03 |
-1.24 |
| 2 |
Suzuki, Mako (12)
|
MRYMID |
33.01 |
33.21 |
0.20 |
| 3 |
Lee, Meredith I (11)
|
MRBGNW |
34.88 |
34.21 |
-0.67 |
| 4 |
Slocum, Annie T (11)
|
MRSMC |
37.23 |
35.97 |
-1.26 |
| 5 |
Hendrickson, Riley D (11)
|
MRYMID |
36.49 |
36.31 |
-0.18 |
| 6 |
Mejia, Jazmine (12)
|
MRYMID |
36.06 |
36.58 |
0.52 |
| 7 |
Avelino, Jazmin (11)
|
CTZEUS |
38.27 |
36.95 |
-1.32 |
| 8 |
Chernishova, Maria E (12)
|
MRSHY |
39.33 |
37.07 |
-2.26 |
| 9 |
Zecar, Eva D (12)
|
MRYFD |
42.00 |
38.00 |
-4.00 |
| 10 |
Jimenez, Hilary J (12)
|
CTZEUS |
38.87 |
38.30 |
-0.57 |
| 11 |
Burdzicki, Victoria M (12)
|
CTZEUS |
39.79 |
38.79 |
-1.00 |
| 12 |
VanVelzor, Hannah M (11)
|
CTZEUS |
38.67 |
39.83 |
1.16 |
| 13 |
Stillman, Alexandra B (11)
|
MRYFD |
45.85 |
40.33 |
-5.52 |
| 14 |
McCann, Katherine E (11)
|
MRYMID |
40.37 |
40.61 |
0.24 |
| 15 |
Malone, Lily R (11)
|
CTZEUS |
38.66 |
40.84 |
2.18 |
| 16 |
Davidson, Freja V (11)
|
MRRA |
46.16 |
41.81 |
-4.35 |
| 17 |
Goyal, Arya (11)
|
MRYMID |
48.05 |
43.26 |
-4.79 |
| 18 |
Herman, Ava S (11)
|
MRSMC |
57.99 |
46.40 |
-11.59 |
| 19 |
Le, Charlotte (12)
|
MRYFD |
|
48.84 |
- |
| 20 |
King, Kayla S (11)
|
MRBGNW |
50.98 |
52.77 |
1.79 |
| 21 |
Lookstein, Olivia R (11)
|
MRYFD |
|
53.41 |
- |
|
Amerson, Sydney D (11)
|
MRSMC |
35.45 |
DQ |
- |
|
Clare, Maya I (11)
|
MRYFD |
|
DQ |
- |
|
Feng, Summer (11)
|
MRYMID |
|
NS |
- |